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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
05/12/2018 |
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Actualizado : |
26/08/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
RUBIO, L.; HERNÁNDEZ, L.; BENÍTEZ, M. J.; ARRUABARRENA, A.; RIVAS, F.; COLINA, R.; MAESO, D. |
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Afiliación : |
LETICIA PAOLA RUBIO CATTANI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LESTER HERNÁNDEZ RODRÍGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.; MARÍA JOSÉ BENÍTEZ GALEANO, Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte (CENUR- Litoral Norte), Universidad de la República, Uruguay; ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS FERNANDO RIVAS GRELA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODNEY COLINA, Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte (CENUR- Litoral Norte), Universidad de la República, Uruguay; DIEGO CESAR MAESO TOZZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Biological and molecular characterization of Uruguayan citrus tristeza virus field isolates. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Journal of Plant Pathology, 2019, volume 101, Issue 1, pages 97-105. |
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ISSN : |
1125-4653 // Online ISSN 2239-7264 |
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DOI : |
10.1007/s42161-018-0149-0 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 06 June 2018 // Accepted 29 July 2018 // First Online 13 August 2018 // Published 15 February 2019.
Acknowledgements: This research was funded by Instituto Nacional de Investigación Agropecuaria, Uruguay (project CT-06), through the National Program of Citrus, in collaboration with the Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte de la Universidad de la República.
Funding: This study was funded by Instituto Nacional de Investigación Agropecuaria, Uruguay (project CT-06). |
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Contenido : |
ABSTRACT.
Citrus tristeza virus (CTV) is the causal agent of the most important viral disease of citrus. Symptoms that may affect the productive potential of citrus plants are observed in Uruguayan orchards even though resistant rootstocks are used. CTVis fully eliminated in propagative materials by the National Sanitation and Certification Program, but since the virus and its vector are widespread in the country, the risk of infection in the field persists. In this situation, using mild CTV strains in a cross-protection program would be a useful alternative to attempt to increase yield and quality of the local citrus industry. To this aim, this study assessed the biological and molecular characteristics of 32 local CTV isolates. Bioassays were conducted in a greenhouse with controlled conditions. Each isolate was graft-inoculated on Mexican lime, sweet orange, sour orange and Duncan grapefruit indicator plants. Symptoms and their intensity were evaluated. Molecular characterization was carried out by RT-PCR amplification, using primers for the p25, p20 and p23 genes. PCR products were sequenced, nucleotide sequences were aligned with international reference strains and phylogenetic trees were constructed. Results of the biological and molecular analysis showed the prevalence of severe CTV isolates with a high genetic variability. Two out of 32 characterized isolates were selected as mild CTV isolates to be tested as candidates for future cross-protection experiments. The survey showed a complex scenario for the management of CTV in Uruguay.
© Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2018 MenosABSTRACT.
Citrus tristeza virus (CTV) is the causal agent of the most important viral disease of citrus. Symptoms that may affect the productive potential of citrus plants are observed in Uruguayan orchards even though resistant rootstocks are used. CTVis fully eliminated in propagative materials by the National Sanitation and Certification Program, but since the virus and its vector are widespread in the country, the risk of infection in the field persists. In this situation, using mild CTV strains in a cross-protection program would be a useful alternative to attempt to increase yield and quality of the local citrus industry. To this aim, this study assessed the biological and molecular characteristics of 32 local CTV isolates. Bioassays were conducted in a greenhouse with controlled conditions. Each isolate was graft-inoculated on Mexican lime, sweet orange, sour orange and Duncan grapefruit indicator plants. Symptoms and their intensity were evaluated. Molecular characterization was carried out by RT-PCR amplification, using primers for the p25, p20 and p23 genes. PCR products were sequenced, nucleotide sequences were aligned with international reference strains and phylogenetic trees were constructed. Results of the biological and molecular analysis showed the prevalence of severe CTV isolates with a high genetic variability. Two out of 32 characterized isolates were selected as mild CTV isolates to be tested as candidates for future cross-protection experiments. The su... Presentar Todo |
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Palabras claves : |
BIOLOGICAL INDEXING; CITRUS TRISTEZA VIRUS (CTV); CLOSTEROVIRIDAE; CLOSTEROVIRUS; PHYLOGENETIC ANALYSIS. |
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Thesagro : |
CITRUS. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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Marc : |
LEADER 03065naa a2200301 a 4500
001 1059343
005 2019-08-26
008 2019 bl uuuu u00u1 u #d
022 $a1125-4653 // Online ISSN 2239-7264
024 7 $a10.1007/s42161-018-0149-0$2DOI
100 1 $aRUBIO, L.
245 $aBiological and molecular characterization of Uruguayan citrus tristeza virus field isolates.$h[electronic resource]
260 $c2019
500 $aArticle history: Received 06 June 2018 // Accepted 29 July 2018 // First Online 13 August 2018 // Published 15 February 2019. Acknowledgements: This research was funded by Instituto Nacional de Investigación Agropecuaria, Uruguay (project CT-06), through the National Program of Citrus, in collaboration with the Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte de la Universidad de la República. Funding: This study was funded by Instituto Nacional de Investigación Agropecuaria, Uruguay (project CT-06).
520 $aABSTRACT. Citrus tristeza virus (CTV) is the causal agent of the most important viral disease of citrus. Symptoms that may affect the productive potential of citrus plants are observed in Uruguayan orchards even though resistant rootstocks are used. CTVis fully eliminated in propagative materials by the National Sanitation and Certification Program, but since the virus and its vector are widespread in the country, the risk of infection in the field persists. In this situation, using mild CTV strains in a cross-protection program would be a useful alternative to attempt to increase yield and quality of the local citrus industry. To this aim, this study assessed the biological and molecular characteristics of 32 local CTV isolates. Bioassays were conducted in a greenhouse with controlled conditions. Each isolate was graft-inoculated on Mexican lime, sweet orange, sour orange and Duncan grapefruit indicator plants. Symptoms and their intensity were evaluated. Molecular characterization was carried out by RT-PCR amplification, using primers for the p25, p20 and p23 genes. PCR products were sequenced, nucleotide sequences were aligned with international reference strains and phylogenetic trees were constructed. Results of the biological and molecular analysis showed the prevalence of severe CTV isolates with a high genetic variability. Two out of 32 characterized isolates were selected as mild CTV isolates to be tested as candidates for future cross-protection experiments. The survey showed a complex scenario for the management of CTV in Uruguay. © Società Italiana di Patologia Vegetale (S.I.Pa.V.) 2018
650 $aCITRUS
653 $aBIOLOGICAL INDEXING
653 $aCITRUS TRISTEZA VIRUS (CTV)
653 $aCLOSTEROVIRIDAE
653 $aCLOSTEROVIRUS
653 $aPHYLOGENETIC ANALYSIS
700 1 $aHERNÁNDEZ, L.
700 1 $aBENÍTEZ, M. J.
700 1 $aARRUABARRENA, A.
700 1 $aRIVAS, F.
700 1 $aCOLINA, R.
700 1 $aMAESO, D.
773 $tJournal of Plant Pathology, 2019, volume 101, Issue 1, pages 97-105.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
13/04/2023 |
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Actualizado : |
13/04/2023 |
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Tipo de producción científica : |
Software |
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Autor : |
KASPARY, T. E.; GARCIA, A.; MARQUES, S.; CABRERA, M. |
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Afiliación : |
TIAGO EDU KASPARY, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MILTON ALEJANDRO GARCIA LATASA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SOFÍA MARQUES HILL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ORLANDO MAURICIO CABRERA GARCIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
HRAC Group 9 (Legacy G) Resistant Palmer Amaranth (Amaranthus palmeri). Inhibition of Enolpyruvyl Shikimate Phosphate Synthase HRAC Group 9 (Legacy G), Uruguay, Lavalleja, Soriano. [web database]. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Heap, I. The International Herbicide-Resistant Weed Database. Online. Thursday, April 13, 2023. Available: www.weedscience.org |
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Idioma : |
Inglés |
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Notas : |
Citation: Heap, I. The International Herbicide-Resistant Weed Database. Online. Thursday, April 13, 2023. Available: www.weedscience.org Copyright © 1993- 2023 WeedScience.org All rights reserved. Fair use of this material is encouraged. Proper citation is requested. Acknowledgements: The Herbicide Resistance Action Committee, The Weed Science Society of America, and weed scientists in Uruguay have been instrumental in providing you this information. Particular thanks is given to Mauricio Cabrera, M. Alejandro Garcia, Tiago Edu Kaspary, and Sofia Marques Hill for providing detailed information. |
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Contenido : |
INTRODUCTION: Palmer Amaranth (Amaranthus palmeri) is a dicot weed in the Amaranthaceae family. In Uruguay this weed first evolved resistance to Group 9 (Legacy G) herbicides in 2022 and infests Corn (maize), and Soybean. Group 9 (Legacy G) herbicides are known as Inhibition of Enolpyruvyl Shikimate Phosphate Synthase (Inhibition of EPSP synthase). Research has shown that these particular biotypes are resistant to glyphosate and they may be cross-resistant to other Group 9 (Legacy G) herbicides. Copyright © 1993-2023 WeedScience.org All rights reserved. Fair use of this material is encouraged. Proper citation is requested. |
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Palabras claves : |
Amaranthus palmeri; Palmer Amaranth; WEEDS. |
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Asunto categoría : |
H60 Malezas y escardas |
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URL : |
https://www.weedscience.org/Pages/Case.aspx?ResistID=24254
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Marc : |
LEADER 01980nam a2200193 a 4500
001 1064034
005 2023-04-13
008 2023 bl uuuu u0uu1 u #d
100 1 $aKASPARY, T. E.
245 $aHRAC Group 9 (Legacy G) Resistant Palmer Amaranth (Amaranthus palmeri). Inhibition of Enolpyruvyl Shikimate Phosphate Synthase HRAC Group 9 (Legacy G), Uruguay, Lavalleja, Soriano. [web database].$h[electronic resource]
260 $aHeap, I. The International Herbicide-Resistant Weed Database. Online. Thursday, April 13, 2023. Available: www.weedscience.org$c2023
500 $aCitation: Heap, I. The International Herbicide-Resistant Weed Database. Online. Thursday, April 13, 2023. Available: www.weedscience.org Copyright © 1993- 2023 WeedScience.org All rights reserved. Fair use of this material is encouraged. Proper citation is requested. Acknowledgements: The Herbicide Resistance Action Committee, The Weed Science Society of America, and weed scientists in Uruguay have been instrumental in providing you this information. Particular thanks is given to Mauricio Cabrera, M. Alejandro Garcia, Tiago Edu Kaspary, and Sofia Marques Hill for providing detailed information.
520 $aINTRODUCTION: Palmer Amaranth (Amaranthus palmeri) is a dicot weed in the Amaranthaceae family. In Uruguay this weed first evolved resistance to Group 9 (Legacy G) herbicides in 2022 and infests Corn (maize), and Soybean. Group 9 (Legacy G) herbicides are known as Inhibition of Enolpyruvyl Shikimate Phosphate Synthase (Inhibition of EPSP synthase). Research has shown that these particular biotypes are resistant to glyphosate and they may be cross-resistant to other Group 9 (Legacy G) herbicides. Copyright © 1993-2023 WeedScience.org All rights reserved. Fair use of this material is encouraged. Proper citation is requested.
653 $aAmaranthus palmeri
653 $aPalmer Amaranth
653 $aWEEDS
700 1 $aGARCIA, A.
700 1 $aMARQUES, S.
700 1 $aCABRERA, M.
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